iPSC 重编程与克隆筛选追踪

Reprogramming Efficiency / 重编程效率

ENCalculate efficiency based on starting cell count and colony formation. Essential for optimizing reprogramming methods (Sendai, mRNA, Episomal).

CN基于起始细胞数量和克隆形成数计算重编程效率。对于优化重编程方法（如仙台病毒、mRNA、游离质粒）至关重要。

Formula: (Colonies / Start Cells) × 100%

Clone Tracker / 克隆筛选追踪

ENMonitor passage numbers (P#) to prevent genetic instability. High passage numbers increase the risk of karyotypic abnormalities.

CN监控传代次数（P#）以防止遗传不稳定性。高代次显著增加核型异常的风险。

• P < 15: Healthy / 健康
• P 15-24: Warning / 警告
• P ≥ 25: High Risk / 高危

Differentiation Potential / 分化潜能

ENScore trilineage differentiation capacity (Ectoderm, Mesoderm, Endoderm) to validate pluripotency.

CN对三胚层（外胚层、中胚层、内胚层）分化能力进行评分，以验证细胞的多能性。