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流式细胞术工业工具箱

Flow Cytometry Industrial Suite

v2.1 Pro
2026-01-28

Configuration

Calculated Prep Volume (N'): 11.0 tests

Reagents

Reagent Name µL/Test Cost/Test

Batch Preparation Record

Total Vol: 0 µL
Reagent Vol per Test (µL) Total Vol (µL) Lot # Verify
Cost Per Test: ¥0.00
Batch Total Cost: ¥0.00

Standard Curve Data

Peak MFI (x) MESF/ABC (y)
Peak 1
Peak 2
Peak 3
Peak 4
Peak 5

Regression Analysis

-
-
-
Waiting for data...

-

Receptor Occupancy (Free Receptor Assay)

Calculates % Free and % Occupied receptor based on MFI.

Receptor Occupancy (RO)
- %
Free Receptor: - %

Absolute Counting (Single Platform)

Calculate absolute cell count using counting beads.

-
cells / µL

Industrial Suite User Guide

1. Master Mix Calculator (主混液配单)

Calculate reagent volumes for antibody cocktails. Enter the number of samples (N) and overage percentage. Add reagents with their volumes per test. The tool automatically calculates the total batch volume including overage.

计算抗体混合液的试剂用量。输入样本数量 (N) 和损耗比例 (Overage)。添加试剂及其单次用量,工具会自动计算包含损耗在内的总配制体积。

2. MESF QA/QC (标准曲线)

Generate standard curves using calibration beads (e.g., Quantum MESF). Input MFI and MESF values for at least 2 peaks to calculate slope, intercept, and R². Use the regression to calculate MESF/ABC for unknown samples.

使用校准微球(如 Quantum MESF)生成标准曲线。输入至少 2 个峰的 MFI 和 MESF 值以计算斜率、截距和 R²。利用回归方程计算未知样本的 MESF/ABC 值。

3. Receptor Occupancy (受体占用率)

Calculate Free and Occupied receptor percentages. Requires: Sample MFI (Drug Treated), Max Control MFI (100% Free), and Isotype MFI (0% Free).

计算受体占用率 (RO)。需要输入:给药样本 MFI、最大阳性对照 MFI (100% Free) 和同型对照 MFI (0% Free)。

4. Absolute Count (绝对计数)

Calculate absolute cell counts using single-platform beads (liquid or lyophilized). Supports both "Beads/µL" and "Total Beads/Tube" modes.

使用单平台微球(液体或冻干粉)计算细胞绝对计数。支持“微球/µL”和“单管总微球数”两种模式。

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