产品详情
肿瘤坏死因子α（TNF α）刺激免疫反应的急性期。针对病原体，TNF α是最早释放的物质之一，能够在许多器官中发挥作用。TNF α刺激促皮质释放激素，抑制食欲，并诱发下丘脑发热。TNF 增加血管扩张和血管通透性丧失，通过调节趋化因子释放，帮助将淋巴细胞、中性粒细胞和单核细胞招募到炎症部位。
蛋白序列
MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL with polyhistidine tag at the C-terminus.
产品组分
| 别称 | TNF-α、肿瘤坏死因子α |
| 蛋白编码 | P01375 |
| 蛋白标签 | His Tag (C-term) |
| 表达系统 | Escherichia coli |
| 生物活性 | Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is < 0.1 ng/mL. The specific activity of recombinant human TNF alpha is approximately ≧ 1 x 10⁷ IU/mg. |
| 纯度 | >97% as determined by SDS-PAGE. |
| 内毒素检测 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 分子量 | The protein has a calculated MW of 18.3 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis). |
实验数据
储存条件
冻干蛋白： 在-20°C 下保存一年。
复溶溶液：保存温度为 2°C 至 8°C，最多可保存一周；添加稳定剂，在-20°C 或-80°C 下保存 3 到 6 个月。
运输条件：干冰
